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E4274
Sigma
Endotoxin Removal solution
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Description
| Quantity | 1 volume treats 5 volumes of DNA solution (≤1mg/ml) |
| Biochem/physiol Actions | The reagent relies on the micellar properties of Triton X-114 to remove endotoxins from solution. When warmed above 20 °C, micelles aggregate into a separate phase that sequesters amphiphilic solutes, including lipopolysaccharides. The hydrophilic DNA remains in the aqueous phase. |
Safety
References
| reference | Cotton, M., et al. Gene Ther. 1, 239, (1994) Abstract |
